ProtoCOL

Colony Counters


Features

ProtoCOL

 

 

ProtoCOL is equipped with a unique colony detection system which combines special LED illumination and digital color camera control with powerful software. ProtoCOL comes standard with built in computer to minimize the foot print on lab bench. Computer specifications are updated as new processors and other advanced components become available. ProtoCOL is available in two different models, ProtocCOL SR (standard resolution) or ProtoCOL HR (high resolution). Both systems come complete with a 19” flat screen monitor, keyboard and mouse.

protocol_system_side_1

ProtoCOL SR

ProtoCOL software provides accurate analysis and enumeration of bacterial plates. The system is able to compensate for writing on plates, variations in agar thickness, touching colonies, meniscus effects, spreaders, bubbles, debris, different sized colonies and different colored media. Measuring applications include enumeration of pour, spread and spiral plates, multi well plate analysis and inhibition zone measurement. Typical measuring time is 1 second/ plate. For standard 90mm Petri dishes, the smallest detectable colony is >0.2mm. Higher magnifications are available with an external camera.

Software allows user to automatically set up serial dilution. A series is established and read with the mean count automatically calculated and reported. Users are also provided with detailed colony size statistics. Instant size distribution histograms and tables provide immediate comparisons between plate populations and can be used to highlight colonies of specific sizes. Users can easily create their own size class intervals and store them for reuse. ProtoCOL software also allows users to draw exclude regions, exclude problem areas on a plate and separate touching colonies.

ProtoCOL ensures no transcription errors as results are instantly transferred to a results table in a secured database. Different levels of users are governed by password control, ensuring compliance with quality procedures and GLP. Users who wish to reread previously analyzed samples using automatic or manual methods will automatically have a copy of the original result stored along with the new result. Users can configure ProtoCOL to request a “reason for change” free text comment which is recorded with the data in a comments field. Users can choose to save true color sample images for archive purposes; these images can be printed out directly from ProtoCOL software. One button click automatically transfers results to and Excel spreadsheet.

ProtoCOL SR Close Up
ProtoCOL SR Software
Zone Sizing For Antibiotics ProtoCOL ProtoCOL Spiral Plate ProtoCOL Plate ProtoCOL Plate ProtoCOL Plate

ProtoCOL HR

ProtoCOL HR employs all the features and benefits of the standard ProtoCOL system with the added benefit of a high resolution camera. The HR version has 4 times the resolution of ProtoCOL SR. For standard 90mm Petri dishes, the smallest detectable colony is >0.1mm. Higher magnifications are available with an external camera.

ProtoCOL HR is ideal for applications where the enumeration of micro colonies and / or fine measurement of inhibition zones are required. Typical applications for ProtoCOL HR include enumeration of water filtration samples, samples where typical colonies sizes are less than 100 microns and automated inhibition zone measuring (disk diffusion or drop method).

ProtoCOL HR And Monitor

Specs

 

ProtoCOL SR
Measurement time 0.9 seconds
Resolution 0.2mm (for standard 90mm Petri dishes)
Illumination Upper incident light, bottom transmitted light and dark field using LED arrays
Measurement modes Pour plate, spiral plate, inhibition zones, antibacterial susceptibility
Detection Fully automatic with manual override
Count modes Separation of touching colonies, shape discrimination, area exclusion, colony inclusion/exclusion
Dilution series User definable
Results Display, storage and printing of up to 20 data items for each sample
Image storage/Retrieval Storage of >20,000 images on local hard disk
External connections Mouse, keyboard, bar code reader, monitor, external camera, ethernet
Storage Hard disk and floppy disk
Camera Zero distortion color digital with variable shutter speed
Camera lens 6mm high resolution f 1.2 - closed
Power input 110V 50/60 hz
Weight 15 Kg
Dimensions 452mm X 380mm X 452mm
Included computer specifications Windows XP Professional OS
min. 2.0Ghz processor
min. 20Gb Hard drive
USB connection
1200 X 1600 True Color Graphics display
Floppy drive
ProtoCOL HR
Measurement time 0.9 seconds
Resolution 0.070mm
Illumination Upper incident light, bottom transmitted light and dark field using LED arrays
Measurement modes Pour plate, spiral plate, inhibition zones, antibacterial susceptibility
Detection Fully automatic with manual override
Count modes Separation of touching colonies, shape discrimination, area exclusion, colony inclusion/exclusion
Dilution series User definable
Results Display, storage and printing of up to 20 data items for each sample
Image storage/Retrieval Storage of >20,000 images on local hard disk
External connections Mouse, keyboard, bar code reader, monitor, external camera, ethernet
Storage Hard disk and floppy disk
Camera Zero distortion color digital with variable shutter speed
Camera lens 8.5mm high resolution f 1.2 - closed
Power input 110V 50/60 hz
Weight 15 Kg
Dimensions 452mm X 380mm X 452mm
Included computer specifications Windows XP Professional OS
min. 2.0Ghz processor
min. 20Gb Hard drive
USB connection
1200 X 1600 True Color Graphics display
Floppy drive

Accessories

Color differentiation software module:
Differentiates up to seven different colony types based on color. Useful with chromogenic media.


Standard resolution (SR) external camera with lens:
For counting larger or multi-well plates; allows for adjustable focal length, as opposed to the fixed focal length for 90mm plates that is standard for ProtoCOL.


High resolution (HR) external camera with lens:
4 times the resolution of the standard resolution external camera


Lighted macro stand:
For use with external camera. Allows viewing of large plates.
Provides lighting for external camera


 

C – mount for external camera connection to microscope

ProtoCOL C-Mount For External Camera To Scope


Customized plate holder
Requires sample blank Petri dish for manufacturing


Bar code reader
For instant reading and input of plate ID


Automated X - Y stage
For precise multi well analysis and enumeration


AutoZone automatic stage
For the analysis of large bioassay / multi-well plates

ProtoCOL Autozone Module

 









 

 

Protocol Darkroom (UV and White light Illumination):
The Protocol Darkroom option allows for direct enumeration and analysis of fluorescent entities (i.e. GFP assay). Benefits include, direct connection to the Protocol system, short and long wavelength UV trans/epi illumination and white light trans/epi illumination. This turnkey system offers an elegant solution to working with UV in a closed and safe environment.

ProtoCOL Darkroom

 

 

Related Products

WASP2 Spiral Plater

WASP 2 Spiral Plater

The WASP 2 spiral plater eliminates the need for serial dilutions, offers a reduction in cost per test and standardizes counting methods. The WASP 2 is fully microprocessor controlled and allows depositions onto 10cm and 15cm plates in logarithmic volume...

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